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Localized inhibition of microtubule polymerization
Localized inhibition of microtubule polymerization
RPOC can inhibit chemical processes at selected locations. For example, pairing with a photoswitchable inhibitor PST-1, RPOC can inhibit microtubule polymerization only in ER, or only in selected cells within a population, without affecting unwanted locations. These studies can be found here:
https://onlinelibrary.wiley.com/doi/10.1002/advs.202307342 Link
Organelle-specific ROS generation
Organelle-specific ROS generation
RPOC can generate ROS in selected organelles and quantify the laser dosage received by each cell. The dosage can be well correlated to cell responses.
This study can be found here: https://onlinelibrary.wiley.com/doi/10.1002/advs.202307342
Selective organelle perturbation and ROS generation
Selective organelle perturbation and ROS generation
RPOC permits selective organelle perturbation using lasers and the generation of ROS solely in desired organelles. The interactive software allows to outline any cells. The signal-based APX selection permits automated target identification with optimal spatial precision. The generation of ROS in mitochondria induces leaking of MitoTracker into cytosol and oxidation of cellular EGFP. This study can be found here: https://www.biorxiv.org/content/10.1101/2024.02.09.579709v1
Simultaneous Creation of different treatment conditions
Simultaneous Creation of different treatment conditions
RPOC can simultaneously create different treatment conditions within the same field of view (FOV). This allows enhancement of the throughput and the study on how cells treated differently interact with each other.
RPOC also enables fixed laser dosages for treatment of desired targets, or automatic stop of treatment after reaching a desired signal level. For example, it can photobleach fluorophores to any defined level and then automatically stop the photobleaching process.
These studies can be found here:
Adaptive photobleaching
Adaptive photobleaching
RPOC offers adaptive and fully-automated photobleaching. It allows to define any pattern or automatically trace any complex molecular distribution from the sample. It allows for the enhanced understanding of protein dynamics. Studies can be found here: https://www.biorxiv.org/content/10.1101/2024.02.09.579709v1
Control of cell division
Control of cell division
RPOC can control cell division and force cells to divide into the multi-nuclei form by perturbing centrosomes using a 405 nm laser. We can control the fate of cells after site-specific and chemical-specific optical control. The cell fate can be monitored in long-term to determine the impact of optical control. This study can be found here: https://www.biorxiv.org/content/10.1101/2024.02.09.579709v1
Control chemical states in live cells
Control chemical states in live cells
RPOC allows to site-specifically control states of photochromic molecules with submicron precision. As an example, a photoswitchable molecule cis−1,2-dicyano1,2-bis(2,4,5-trimethyl-3-thienyl)ethene (CMTE) is used. This study can be found here: https://www.nature.com/articles/s41467-022-32071-z
Raman activated cell-sorting
Raman activated cell-sorting
RPOC allows to perform Raman activated sorting of bacteria with high speed and specificity
https://www.biorxiv.org/content/10.1101/2023.10.16.562526v1.full
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