The first RPOC developed (based on SRS microscopy)

M.G. Clark, G. Gonzalez, Y. Luo, J. Aldana-Mendoza, M.S. Carlsen, G. Eakins, M. Dai, and C. Zhang*. "Real-time precision opto-control of chemical processes in live cells". Nature Communications, 13, 4343 (2022).

 

CW-RPOC (Compatible with confocal fluorescence microscopes)

B. Dong#, S. Mahapatra#, M.G. Clark, et al. and C. Zhang*. "Spatiotemporal precise optical manipulation of intracellular molecular activities", Advanced Science, 2307342 (2024)

 

Software-assisted RPOC (Significantly improved flexibility and throughput)

B. Dong, R. M. Everly, S. Mahapatra, M. S. Carlsen, S. Ma, and C. Zhang*. "Spatially and Chemically Specific Optical Control of Cells via Supervised and Automated Target Selection". ACS Photonics, 12, 3421–3434 (2025)

 

Femtosecond-RPOC (Improved axial precision)

S. Ma, B. Dong, M.G. Clark, R.M. Everly, S. Mahapatra, and C. Zhang*. "Real-Time and Site-Specific Perturbation of Dynamic Subcellular Compartments Using Femtosecond Pulses". Small Science, 2500166 (2025).

 

Applications

S. Mahapatra, S. Ma, B. Dong, and C. Zhang*. "Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization" VIEW, 20240013 (2024).

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J. Zhang, H. Lin, J. Xu*, M Zhang, X. Ge, C. Zhang, W. E. Huang*, and J. X. Cheng*. "High-throughput single-cell sorting by stimulated Raman-activated cell ejection", Science Advances, 10, eadp2438 (2024).

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Reviews and Perspectives

C. Zhang*, B. Dong, S. Mahapatra, S. Ma. "Advances and Future Trends in Real-Time Precision Opto-Control (RPOC) of Chemical Processes in Live Cells". (Review) npj Imaging, 3, 23 (2025).

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M.G. Clark, S. Ma, S. Mahapatra, K. Mohn, and C. Zhang*. "Chemical-imaging-guided optical manipulation of biomolecules", (Perspective) Frontiers in Chemistry. 11:1198670 (2023). Link