RPOC technology
The next generation optical regulation
RPOC stands for Real-Time Precision Opto-Control. It enables unprecedented flexibility, spatial precision, and chemical selectivity for regulating chemical processes within live biological samples.
RPOC can be integrated into any custom laser-scanning microscopes or stage-scanning microscope platforms.
Sentry IV is our most advanced RPOC system based on confocal fluorescence. It offers state-of-the-art capabilities for precisely modulating biological samples with light and simultaneous monitoring their responses.
Option 1. Standalone RPOC system based on laser scanning confocal microscopy
SENTRY IV
Standalone RPOC system based on confocal fluorescence
KEY FEATURES
Option 2. RPOC modules
Add RPOC functionalities to your lab-built laser scanning microscopy
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Retain all the functions of your original microscope
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Covert it from a passive watching station to an active optical control system
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Low cost compared to standalone RPOC system
Typically includes:
Action lasers
Acousto-optic modulator (AOM) (case dependent)
Optical filters
A comparator circuit box
RPOC software
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All fiber coupled CW-lasers
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Automatic selection of targets of any distribution
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Real-time tracking and opto-control of dynamic targets
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Flexible control of laser dose at any targetIntegrated with two channel comparator circuit
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Optoelectronic feedback within 70 ns
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Advanced LabVIEW or Python-based opto-control software
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Simultaneous opto-control and monitoring of cell responses from seconds to days*
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All functionalities of a confocal fluorescence microscope**
*If a stage top incubator is installed
**Standard version comes with 4 lasers and 2 PMTs
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Spatial precision: <300 nm
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Response time: 70-500 ns
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Action laser: Fiber-coupled CW lasers (375 nm, 405 nm, 532 nm, etc.), femtosecond NIR lasers
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Chemical detection/readout: Fluorescence, Raman, IR, etc.
Lab-VIEW or Python based RPOC software
RPOC prototype used by our collaborator
“RPOC’s ability to image and stimulate at the same time, especially for in vivo wounding and optogenetic stimulation, enables a temporal resolution which is irreplaceable for our studies of early tissue-scale wound response.”
—— Shelly Tan, Biologist,
Purdue University Department of Biological Science