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  • Two channel comparator circuit for high-speed analog signal comparison with thresholds

  • Invert functions available

  • Output TTL=1, TTL=0, and comparator output available

  • Manual or digital threshold input

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The comparator circuit box performs real-time comparison of optical signals against a user-defined threshold and generates TTL outputs accordingly. It also offers direct TTL 1 and 0 outputs for controlling common lab instruments. 

 

It is well-suited for Real-Time Precision Opto-Control (RPOC) applications, including biomolecular control, cell sorting, and integration with other modalities for real-time decision-making.

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  • Two separate comparator circuits

  • Use independently or in combination

  • Digital logic functions (AND, OR, NOT) available

  • Manual tuning or digital/analog input of voltage threshold

  • Fast response timeMultiple parallel output

How to add RPOC to your laser scanning microscopy systems with the comparator circuit?

Adding RPOC functionality to your microscope is straightforward. All you need is a comparator circuit and, optionally, RPOC control software.

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Additionally, you can select from a variety of laser sources to serve as action lasers. The comparator circuit is typically used in conjunction with an acousto-optic modulator (AOM) to deflect the action laser, or it can directly control the laser itself.

Real-time mitochondria laser perturbations using the comparator circuit box and real-time precision opto-control (RPOC)

MitoTracker leakage from mitochondria

405 nm lasers pertrub solely mitochondria

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References:

Clark, et al. Nat Commun, 2022, 13, 4343

Dong, et al. Adv Sci, 2024, 2307342

Ma, et al. Small Sci, 2025, 2500166

Dong, et al. ACS Photonics, 12, 7, 3421-3434

Singh, et al. Chem Methods, 6, e202500153

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